Composite

Part:BBa_K4307028:Design

Designed by: Chi Zhang   Group: iGEM22_Tsinghua   (2022-09-30)


pT7-AffiPmrB-PmrA-PmrC-EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 388
    Illegal PstI site found at 676
    Illegal PstI site found at 1108
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1855
    Illegal PstI site found at 388
    Illegal PstI site found at 676
    Illegal PstI site found at 1108
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 939
    Illegal BamHI site found at 2283
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 388
    Illegal PstI site found at 676
    Illegal PstI site found at 1108
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 388
    Illegal PstI site found at 676
    Illegal PstI site found at 1108
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

waiting


Source

E.coli

References